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Metabolic Changes in Mouse Plasma after Acute Diquat Poisoning by UPLC-MS/MS

Author(s):

Lianguo Chen, Zuoquan Zhong, Jiawen Liu, Congcong Wen, Yongxi Jin and Xianqin Wang*   Pages 1 - 5 ( 5 )

Abstract:


Introduction: Diquat is a fast-acting contact herbicide and plant dehydrating agent. The oral lethal dose 50 (LD50) of diquat in mice is about 125 mg/kg. The purpose of this study is to research the metabolomics in mouse plasma after acute diquat poisoning.

Method: These mice were divided into two groups (the control group and acute diquat poisoning group). The control group was given normal saline by gavage. The acute diquat poisoning group was given 50 mg/kg diquat. UPLC-MS/MS was used to determinate the small molecule organic acid in mouse plasma.

Results: Compare to the control group, the L-lysine, Adenine, L-Alanine, L-Valine, Lactic acid, Inosine, Adenosine, LTryptophan, L-Tyrosine, L-Arginine, L-Phenylalanine, L-Methionine, Citric acid, Fructose, L-Glutamine, Malic acid, LAspartic acid and Pyruvic acid increased in the acute diquat poisoning group (p<0.05); while the L-Histidine decreased (p<0.05).

Conclusion: The results of metabolites increased or decreased, indicating that acute diquat poisoning induced amino acid metabolism and energy metabolism perturbations in mice.

Keywords:

Diquat, plasma, UPLC-MS/MS, amino acid, mouse, metabolomics

Affiliation:

The Third Clinical Institute Affiliated with Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Department of Rehabilitation, Wenzhou Municipal Hospital of Traditional Chinese Medicine, Wenzhou 325005, Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035



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