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Chiral Resolution and Content Determination of Ketorolac Tromethamine using High-performance Liquid Chromatography

[ Vol. 17 , Issue. 10 ]

Author(s):

Lei Zheng*, Jing Yang, Yu-yao Guan, Lei Zhang, Chao Song* and Xiao-li Zhang*   Pages 1257 - 1262 ( 6 )

Abstract:


Background: Establishing R, S-enantiomer (S-KT and R-KT) chiral resolution and determination methods for KT are of great significance.

Objective: This study aimed to establish a high-performance liquid chromatography (HPLC) method for the resolution and determination of ketorolac tromethamine (KT) enantiomers.

Methods: A CHIRALPAK AGP column (0.4 × 10 cm, 5 μm) was used with 10 mmol/L ammonium acetate (pH 5.5) and isopropanol (97:3) as the mobile phase. The detection wavelength was 324 nm, the flow rate was 1.0 mL/min, the column temperature was 25°C, and the injection volume was 5 μL.

Results: The resolution between S-KT and R-KT was 2.8. S-KT and R-KT demonstrated a good linear relationship in the range of 3-60 μg/mL (r > 0.999). The average recoveries of S-KT and RKT were 99.2% and 99.8%, with relative standard deviations of 2.0% and 2.4%, respectively.

Conclusion: The established method can be used for the resolution and determination of S-KT and R-KT.

Keywords:

Ketorolac tromethamine, enantiomers, HPLC, chiral resolution, determination, respiratory depression.

Affiliation:

School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, Departement of Pharmacy, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250031, Linyi Institute of inspection and Testing Center, Linyi, Shandong, 276000,, Departement of Pharmacy, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250031, Departement of Pharmacy, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250031

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