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Determination of Acotiamide in Human Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study

Author(s):

Qian Sun, Qiao-gen Zou*, Yun-yan Xia and Cheng-qun Han   Pages 1 - 8 ( 8 )

Abstract:


Background: A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method had been developed for the quantification of acotiamide in rat plasma and been applied to pharmacokinetic studies. However, there was no LC-MS/MS method been developed for the determination of acotiamide in human plasma and its pharmacokinetic study.

Objective: A simple and fast LC-MS/MS method was established and validated for the quantification of acotiamide in human Received: plasma and was applied to a pharmacokinetic study.

Methods: Sample preparation was accomplished Revised: Accepted: through protein precipitation, and chromatographic separation was achieved on a Welch, Ultimate XB-C18 column (2.1×50 mm, 3 μm) with a security guard cartridge C18 using a binary gradient with DOI: mobile phase A (Methanol) and B (the solution of 10 mM Ammonium acetate with 0.1% Formic acid) at a flow rate of 400

Keywords:

Acotiamide, LC-MS/MS, human plasma, chromatographic separation, protein precipitation, pharmacokinetic

Affiliation:

Department of Pharmaceutical Sciences, Nanjing Technology University, Nanjing 210009,, Department of Pharmaceutical Sciences, Nanjing Technology University, Nanjing 210009,, Department of Pharmaceutical Sciences, Nanjing Technology University, Nanjing 210009,, Department of Pharmaceutical Sciences, Nanjing Technology University, Nanjing 210009



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