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Comparison of HPLC-DAD and UPLC-MS/MS in monitoring serum concentration of lamotrigine

Author(s):

Xubin Wang*, Zhibin Chen , Xiaofan Ke, Yingying Wang, Lufeng Hu* and Congrong Tang*  

Abstract:


Background: Lamotrigine (LTG) is a broad-spectrum and first-line anti-epileptic drug. To monitor the serum levels of LTG in epileptic seizures patients, high-performance liquid chromatography with diode-array detection (HPLC-DAD) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) methods were established and compared.

Methods: Imatinib was used as the internal standard (IS) for both methods. LTG and IS were detected at 246 nm by HPLC-DAD. In UPLC-MS/MS, LTG and IS positive ion were detected by multiple reaction monitoring (MRM), with m/z of 256/210.9 and 494/394.02, respectively. A total of 37 blood samples from epileptic patients were determined and studied by these two methods.

Results: There was an acceptable linearity for the two methods. The concentration range of LTG was 0.59 ~ 22.20 mg/L by HPLC, and 0.28 ~ 23.97 mg/L by UPLC-MS/MS.The Pearson regression coefficient of Deming regression was 0.9653 (95% CI: 0.9332 to 0.9821). Bland–Altman method demonstrated that the concentration of LTG determined by UPLC-MS/MS was 8.3% higher than that determined by HPLC (limits of agreement, -32.0% to +48.6%).

Conclusion: There was a significant correlation between the two methods. Both HPLC and UPLC-MS/MS can be used for routine clinical monitoring of LTG.

Keywords:

Lamotrigine, HPLC, UPLC-MS/MS, Determination, Serum, Precipitation

Affiliation:

Department of Neurology, The Affiliated Yueqing Hospital of Wenzhou Medical University, Yueqing, Zhejiang, Department of Nephrology, The Affiliated Yueqing Hospital of Wenzhou Medical University, Yueqing, Zhejiang, Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000



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