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Permeation of Hydroxypropyl-Beta-Cyclodextrin and Its Inclusion Complex Through Mouse Small Intestine Determined by Spectrophotometry

Author(s):

Ping Yang, Jinhua Luo, Shuo Yan, Xiaohong Li and Qian Yao*   Pages 1 - 9 ( 9 )

Abstract:


Background: Cyclodextrins (CDs) are commonly used host molecules of inclusion complex. However, due to the lack of a sensitive determination method, the absorption process of CDs remains unclear.

Objective: In this study, an oleuropein (OL) inclusion complex employing hydroxylpropyl-betacyclodextrin (HP-beta-CD) as host molecules was prepared and the formation of inclusion complex was ascertained by FT-IR and DSC. Spectrophotometry was established for the determination of HP-beta-CD, based on the fact that the absorbance of phenolphthalein (PP) decreased in the presence of HP-beta-CD.

Methods: The assay conditions were optimized to augment the method sensitivity. Molecular docking was employed to verify the strong interaction between PP and HP-beta-CD. The permeation process of free HP-beta-CD, HP-beta-CD of OL inclusion complex, free OL, and OL in the inclusion complex, was examined using an in vitro mouse small intestine model.

Results: Though HP-beta-CD possessed a hydrophilic outside shell, it could permeate through the mouse small intestine quickly with a cumulative permeating amount of over 90% in 2 h. Free HPbeta- CD, the host molecule HP-beta-CD, and guest molecule OL of the inclusion complex exhibited consistent permeating profiles across the mouse small intestine.

Conclusion: The approach for the determination of HP-beta-CD was accurate and precise (%RSD=2.98).

Keywords:

Hydroxylpropyl-beta-cyclodextrin, oleuropein, inclusion complex, phenolphthalein, spectrophotometry, intestinal permeation.

Affiliation:

Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, School of Basic Medicine, Chengdu University, Chengdu 610106, School of Food and Bioengineering, Chengdu University, Chengdu 610106, Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106



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