Submit Manuscript  

Article Details


NMR-based Metabolomic Techniques Identify the Anticancer Effects of Three Polyphyllins in HepG2 Cells

Author(s):

Feng Su*, Haibo Wang, Yifan Wang, Lv Ye, Peixi Zhu, Jinping Gu and Weike Su*   Pages 1 - 12 ( 12 )

Abstract:


Background:Rhizoma Paridis (RP) is a traditional Chinese herb used for the treatment of tumors, detoxification and hemostasia. Studies show the main components of RP are Polyphyllin I (PPI), polyphyllin VI (PPVI), and polyphyllin VII (PPVII). However, the pharmaco-mechanisms of these compounds are not clear.

Objective:By used 1H nuclear magnetic resonance (1H-NMR) based metabolomics approach to identify the Anticancer effects of PPI, PPVI and PPVII in HepG2 cells.

Methods 1H nuclear magnetic resonance (1H-NMR) based metabolomics approach was applied to investigate the toxicological effect of PPI, PPVI, PPVII on HepG2 cells. Multivariate statistical analysis was employed to examine the metabolic changes and abnormal metabolic pathways, including Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA), and orthogonal PLS-DA (OPLS-DA).

Results:The results showed that the effects of metabolic phenotypes were affected separately by PPI, PPVI, and PPVII. The metabolic phenotypes were also changed over time. The characteristic metabolites were varied by affecting different polyphylins, which were identified by the reconstructed OPLSDA loading plots. According to the characteristic metabolites, the mainly disturbed metabolic pathways were found, such as alanine, aspartate and glutamate metabolism, pyruvate metabolism, glycine, serine, and threonine metabolism.

Conclusion:The current work could allow us to understand the therapeutic effect of RP in metabolism. It also indicated that RP would be a promising candidate for liver cancer treatment.

Keywords:

Polyphyllin, nuclear magnetic resonance, metabolomics, metabolic analysis, HepG2 cell, PCA.

Affiliation:

College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000, Zhejiang Hongyuan Pharmaceutical Co., Ltd., Linhai 317000, College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000, Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000, Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000, College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000, College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310000



Read Full-Text article