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A Sensitive Chromatographic Method for Hyaluronate Quantification Applied to Analyze the Desorption Behavior on Contact Lenses

[ Vol. 16 , Issue. 6 ]

Author(s):

Wan-Hsin Chang, Pei-Yi Liu, Yuan-Ting Jiang* and Yuan-Hao Howard Hsu*   Pages 782 - 791 ( 10 )

Abstract:


Background: Sodium hyaluronate (NaHA) is generally supplemented in products related to contact lenses for increasing comfort during wearing. The quantity of sodium hyaluronate and the material of lenses affect the retention of sodium hyaluronate on the contact lenses.

Methods: We developed a convenient and sensitive but unconventional chromatographic method to quantify sodium hyaluronate and analyze its release behavior from contact lenses. The reverse-phase chromatography eluted sodium hyaluronate with high molecular masses in the shortest time and could separate salt and small compounds from sodium hyaluronate.

Results: This method could accurately quantify sodium hyaluronate with diverse molecular sizes. Because sodium hyaluronate was eluted in a narrow time frame, sensitivity was significantly enhanced, and the limit of detection of this method was 0.45 μg/mL. According to this quantitation method, the attached quantity of sodium hyaluronate is related to the water content of the material. Furthermore, a material test indicated that the release efficiency of sodium hyaluronate depends on the material of lenses. Nonionic Polymacon had a longer half-life in the sodium hyaluronate release curve than negative Methafilcon A and silicone hydrogel.

Conclusion: This hyaluronate quantification method is a fast, sensitive and accurate method, making it suitable for the in vitro hyaluronate research without further derivatization.

Keywords:

Sodium hyaluronate, liquid chromatography, contact lenses, methafilcon, polymacon, silicone hydrogel.

Affiliation:

R&D Center, Yung Sheng Optical Co., Taichung, Department of Chemistry, Tunghai University, Taichung, R&D Center, Yung Sheng Optical Co., Taichung, Department of Chemistry, Tunghai University, Taichung

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