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Application of Chemometrics-assisted HPLC-DAD Strategies for Simultaneous Determination of Paracetamol, Pseudoephedrine HCl, Dextromethorphan HBr, Doxylamine Succinate and Saccharin in Syrup Formulation

[ Vol. 17 , Issue. 8 ]

Author(s):

Şule Dinç-Zor, Özlem Aksu Dönmez* and Abdürrezzak E. Bozdoğan   Pages 1043 - 1050 ( 8 )

Abstract:


Introduction: This study introduces an effective strategy, which combines high performance liquid chromatography coupled with diode array detection (HPLC-DAD) with multivariate calibration methods for the simultaneous determination of paracetamol (PAR), pseudoephedrine HCl (PSE), dextromethorphan HBr (DEX) and doxylamine succinate (DOX) along with sweetener saccharin (SAC) in syrup formulation.

Methods: PLS-2 and PCR calibration algorithms were selected for data processing. Based on the strategy, all target analytes were rapidly quantified within 5.3 min under the simple isocratic elution (water: methanol, 20/80, v/v) without a complete separation. The performances of the proposed methods were confirmed by analyzing a series of synthetic solutions including different concentrations of analytes.

Results: The average recovery values were in the range of 100.33 to 103.70%, and the REP (relative error of prediction) values ranged from 1.96 to 4.36% showed that these methods could provide satisfactory predictions.

Conclusion: Novel HPLC methods coupled with PLS and PCR algorithm enable a simple, fast and low-cost analysis of similar pharmaceutical products for simultaneous determination of the target compounds.

Keywords:

Multivariate calibration, HPLC-DAD, multicomponent analysis, saccharin, syrup, paracetamol.

Affiliation:

Department of Chemistry, Faculty of Science and Literature, Yildiz Technical University, Istanbul, Department of Chemistry, Faculty of Science and Literature, Yildiz Technical University, Istanbul, Department of Chemistry, Faculty of Science and Literature, Yildiz Technical University, Istanbul

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