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Investigation and Analytical Applications of the Reaction of N1-methylnicotinamide and Active Methylene Containing Drugs

Author(s):

Heba A. Elsebaie, Mervat H. Elhamamsy, Khaled M. Elokely* and Mohammed A. Eldawy   Pages 1 - 8 ( 8 )

Abstract:


Background: The reaction between N1-methylnicotinamide iodide (NMNI) and the active methylene group containing compounds yields a fluorescent product, which can be used in the quantitative determination of certain drugs.

Objective: The objective is to develop, adapt and validate a simple spectrofluorometric method for the quantitative analysis of modafinil 1, budesonide 2, allicin 3 and florfenicol 4.

Methods: Spectral analysis (elemental analysis, 1H, 13C NMR, MS), computational methods (geometry optimization, Gibbs free energy and pKa calculations) and spectrofluorometric approaches were conducted.

Results: A C4 cyclization pathway was confirmed for the formation of the fluorophore. The spectrofluorometric method showed good linearity (R2= 0.996-0.999) over concentrations of 1-50 ng/mL, 0.5-5 ng/mL, 60-150 pg/mL, and 1-15 ng/mL of the standard solutions of 1, 2, 3, and 4, respectively.

Conclusion: The proposed method is simple and suitable for quality control and assurance studies

Keywords:

Spectrofluorometric analysis; modafinil; budesonide, allicin; florfenicol; free energy calculations.

Affiliation:

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527



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